Genomics Data Repositories

Data Repositories

Getting hold of HTS data

• From public repositories.
• From collaborators.
• By sequencing some of your own material!

class: inverse, center, middle

Repositories for HTS

---

Public Repositories for HTS

• Several public sources of HTS data exist.
• First concentrating on those acting as repositories.
  • GEO (Gene Expression Omnibus).
  • ENA (European Nucleotide Database).
  • SRA (Short Read Archive).

Gene Expression Omnibus

• GEO (https://www.ncbi.nlm.nih.gov/geo/)

• GEO holds different types of biological datasets.
• Very popular for submission of data accompanying publication.
• Captures metadata, processed files and raw data.
• GEO was not built for HTS data.

Gene Expression Omnibus

• GEO (https://www.ncbi.nlm.nih.gov/geo/)

Short Read Archive

• SRA (www.ncbi.nlm.nih.gov/sra)

• NCBI’s HTS specific repository.
• Sequencing specific metadata.
• Stores Raw data (in SRA format)
• SRA format - requires SRA Toolkit

Short Read Archive

• SRA (www.ncbi.nlm.nih.gov/sra)

European Nucleotide Archive

• ENA (https://www.ebi.ac.uk/ena)

• ENA acts as a european HTS repository.
• Mirrors much of SRA.
• Stores Raw data
• No SRA formats - fastq by default.

Other Repositories

• Many repositories contain processed or unprocessed data.
• These typically are the result or a consortium’s data release policies.
• Good example is ENCODE site.
  • (https://www.encodeproject.org/)
• UCSC has many useful links to genomics data in various formats.
  • (http://hgdownload.soe.ucsc.edu/downloads.html)

ENCODE Portal

ENCODE portal provides access to raw and processed/standardised results.

Repositories for processed data

• Other specialist repositories exist.
• ReCount2 database provides standardised counts for user analysis.
• Other databases like Immgen/Bodymap/expression atlas provide RNAseq for specific cells/tissues.

Reference data

• Reference Genome available from many locations.
• Different assemblies.
  • Major Revisisons - Change locations.
  • Minor Revisions - Update annotation.
• Genome sequence stored as FASTA.
• Gene build as GFF3 or GTF.
• IGenomes contains full annotation files for many genomes.