params <- list(isSlides = "no") ## ----include=FALSE------------------------------------------------------------ suppressPackageStartupMessages(require(knitr)) knitr::opts_chunk$set(echo = TRUE, tidy = T) ## ----xaringan-themer, include=FALSE, warning=FALSE, eval=FALSE---------------- ## library(xaringanthemer) ## style_mono_accent( ## base_color = "#23373B", ## title_slide_text_color="white", ## header_font_google = google_font("Montserrat"), ## text_font_google = google_font("Fira Sans", "300", "300i"), ## code_font_google = google_font("Fira Code") ## ) ## ----results='asis',include=TRUE,echo=FALSE----------------------------------- if(params$isSlides != "yes"){ cat("# Cell Ranger --- " ) } ## ----results='asis',include=TRUE,echo=FALSE----------------------------------- if(params$isSlides == "yes"){ cat("class: inverse, center, middle # Set Up

--- " ) }else{ cat("# Set Up --- " ) } ## ----results='asis',include=TRUE,echo=FALSE----------------------------------- if(params$isSlides == "yes"){ cat("class: inverse, center, middle # Cell Ranger

--- " ) }else{ cat("# Cell Ranger --- " ) } ## ----results='asis',include=TRUE,echo=FALSE----------------------------------- if(params$isSlides == "yes"){ cat("class: inverse, center, middle # Cell Ranger

--- " ) }else{ cat("# Cell Ranger --- " ) } ## wget -O cellranger-8.0.0.tar.gz "https://cf.10xgenomics.com/releases/cell-exp/cellranger-8.0.0.tar.gz?Expires=1711772964&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=muvzcbqxba6d-blyYS02MVfLlzwZk6iZNQWXdaoCLnl7owW2nEN-IHwSPwdNoYl-6Xia7rr0S1sLCUQTsekGm2pQKcd0kqK~ndHK0DM7SwSVpXLlRvBV5pXt~EIlsxATVBKVeQLnUy698N-WnRlT~ahjlU-nMdpomX9-lOkF~w8gbgHBdtPXunTWfW87sSJLpHMDVENSF7TFJsXERDwDnsXyQLCuEhfGTCOnupkaATlLEr9kaeCStePKkwGyqgi1m8Ua02NNGHWPIJ6I1mDt695wo~dgptpJF4SDNRTyE-TuXrHfIqRjZB60zhWRJczFo2kpL7FCKwliE-vJ6djcSw__" ## wget "https://cf.10xgenomics.com/supp/cell-exp/refdata-gex-GRCh38-2024-A.tar.gz" ## tar -xzvf cellranger-8.0.0.tar.gz ## tar -xzvf refdata-gex-GRCh38-2020-A.tar.gz ## export PATH=/PATH_TO_CELLRANGER_DIRECTORY/cellranger-8.0.0:$PATH ## cellranger count --id=my_run_name \ ## --fastqs=PATH_TO_FASTQ_DIRECTORY \ ## --transcriptome=/PATH_TO_CELLRANGER_DIRECTORY/refdata-gex-GRCh38-2020-A ## --create-bam=true ## ----results='asis',include=TRUE,echo=FALSE----------------------------------- if(params$isSlides == "yes"){ cat("class: inverse, center, middle # Output files

--- " ) }else{ cat("# Output files --- " ) } ## ----results='asis',include=TRUE,echo=FALSE----------------------------------- if(params$isSlides == "yes"){ cat("class: inverse, center, middle # Web Summary QC

--- " ) }else{ cat("# Web Summary QC --- " ) }